Shin-Ping Lin, Stephanie Singajaya, Tsui-Yun Lo, Shella Permatasari Santoso, Hsien-Yi Hsu, Kuan-Chen Cheng
Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering. © 2023
School of Food Safety, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Research Center of Biomedical Device, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Institute of Biotechnology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Department of Biochemistry and Molecular Cell Biology, School of Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya, 60114, Indonesia; Collaborative Research Center for Sustainable and Zero Waste Industries, Kalijudan 37, East Java, Surabaya, 60114, Indonesia; School of Energy and Environment, Department of Materials Science and Engineering, City University of Hong Kong, Kowloon Tong, 518057, Hong Kong; Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, Hong Kong; Graduate Institute of Food Science and Technology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, 91 Hsueh-Shih Rd., Taichung, 40402, Taiwan; Department of Optometry, Asia University, 500, Lioufeng Rd., Wufeng, Taichung, 41354, Taiwan