Niluh Suwasanti, Cecilia Putri Tedyanto, Silvia Sutandhio, Teguh Hari Sucipto
Dengue infection has become one of the most important mosquito-borne diseases worldwide and is caused by the dengue virus (DENV). Recently, neither patent drug, phytopharmaceutical medicine, nor standardized herbal medicine has been officially available against DENV. Dried red jujube fruit (Ziziphus jujuba) ethanol extract has been proven to have an antiviral effect, anti-inflammatory efficacy, and antioxidant properties, which have potential activity against DENV infection. This research was conducted to analyze the antiviral activity of dried red jujube fruit ethanol extract against DENV-2 in vitro. The half-maximal cytotoxic concentration (CC50) and half-maximal inhibitory concentration (IC50) were examined on Vero cells by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas the half-maximal effective concentration (EC50) was determined using luminescence assay. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. Dried red jujube fruit ethanol extracts inhibited DENV-2 in 24.59%, 22.39%, 17.98%, 14.5%, 6.42%, and 1.28% at 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/ mL, 5 μg/mL, and 2.5 μg/mL, respectively. The extract exhibited antiviral activity against DENV-2, showing a CC50 of 67.73 μg/mL, an IC50 value of 166.18 μg/mL, and an EC50 of 64.87 μg/mL, with an SI of 1.04. The LD50 value was 707.95 mg/kg. Dried red jujube fruit ethanol extract could be a potential candidate for developing an antiviral against DENV-2. © 2024 Phcogj.Com. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Department of Clinical Pathology, Faculty of Medicine, Universitas Katolik Widya Mandala Surabaya, Surabaya, Indonesia; Research Assistant, Faculty of Medicine, Universitas Katolik Widya Mandala Surabaya, Surabaya, Indonesia; Department of Microbiology and Parasitology, Faculty of Medicine, Universitas Katolik Widya Mandala Surabaya, Surabaya, Indonesia; Dengue Study Group, Institude of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia