Shi-Jing Hou, Kuan-Chen Cheng, Shyh-Hsiang Lin, I-Lun Hsiao, Shella Permatasari Santoso, Stephanie Singajaya, Yu-Chieh Chou, Shin-Ping Lin
Cordyceps militaris (C. militaris) is a unique medicinal fungus of the genus Cordyceps. It has high economic value due to various biological functions from the extracellular polysaccharides (EPSs) it produces. The aim of this study was to establish a mycelium-immobilization system for EPS production in a bubble column system. First, EPS production was optimized by 90.2% (from 0.72 to 1.37 g/L) using response surface methodology under an alginate-immobilized mycelium system with 6 days of cultivation. The highest EPS production was obtained (5.3 g/L) in a flask system through elongation to 15 days of cultivation. EPS production subsequently further increased to 10.42 g/L utilizing a bubble column reactor. Furthermore, the system can be reused for at least three rounds. In the EPS characteristics analysis, the produced EPSs were mainly composed of glucose and galactose. They presented strong antibacterial activity against Staphylococcus aureus. In terms of antioxidant activity, respective the EPS concentration that caused 50% scavenging of DPPH and ABTS free radicals values (IC50) were 4.65 and 7.92 mg/mL. In summary, these findings should be helpful for establishing a suitable industrial fermentation system for C. militaris and can be studied in the food and medical industries in future work. © 2024 The Authors
Master Program in Food Safety, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Institute of Biotechnology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Graduate Institute of Food Science and Technology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, 91 Hsueh-Shih Rd., Taichung, 40402, Taiwan; Department of Optometry, Asia University, 500, Lioufeng Rd., Wufeng, Taichung, 41354, Taiwan; School of Nutrition and Health Sciences, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; School of Food Safety, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, East Java, Surabaya, 60114, Indonesia; Collaborative Research Center for Sustainable and Zero Waste Industries, Kalijudan 37, East Java, Surabaya, 60114, Indonesia; Ph.D. Program in Drug Discovery and Development Industry, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Research Center of Biomedical Device, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan