Shin-Ping Lin, Ling Hong, Chen-Che Hsieh, Yun-Hsin Lin, Yu-Chieh Chou, Shella Permatasari Santoso, Chang-Wei Hsieh, Tsung-Yu Tsai, Kuan-Chen Cheng
Owing to a lack of specific biological functions, bacterial cellulose (BC) has been restricted in its application to the field of active packaging. In this study, we developed antimicrobial packaging materials using foaming BC (FBC) with chitosan (CS) and applied it to the preservation of chilled sea bass. The material property analysis demonstrated that 1.5 % CS/FBC maintained a high water content of 91 %, a swelling ratio of 75.6 %, great stress of 1.61 MPa, and great strain of 1.87 %. CS incorporation into FBC also decreased its crystallinity from 73.39 % to 69.3 %. Meanwhile, 1.5 % CS/FBC also provided great antimicrobial ability against Escherichia coli and Staphylococcus aureus by approximately 2 log colony-forming units/mL inhibition utilizing contact-killing. Results of the preservation assessment indicated that 1.5 % CS/FBC efficiently inhibited Shewanella putrefaciens growth, reduced total volatile basic nitrogen release, and slightly inhibited lipid oxidation. Based on the above results, CS/FBC is an ecofriendly biomaterial produced from a microorganism that possesses high absorbency and strong antibacterial properties, making it suitable for development as antibacterial active packaging. © 2024 Elsevier B.V.
School of Food Safety, College of Nutrition, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; TMU Research Center for Digestive Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Research Center of Biomedical Device, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 11031, Taiwan; Institute of Food Science and Technology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Institute of Biotechnology, National Taiwan University, 1 Roosevelt Rd., Sec. 4, Taipei, 10617, Taiwan; Department of Seafood Science, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan; Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, 37 Kalijudan, Surabaya, 60114, Indonesia; Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City, 402, Taiwan; Department of Medical Research, China Medical University Hospital, China Medical University, 91 Hsueh-Shih Road, Taichung, 40402, Taiwan; Department of Food Science, Fu Jen Catholic University, 510 Zhongzheng Rd., Xinzhuang Dist., New Taipei City, 242062, Taiwan; Department of Optometry, Asia University, 500 Lioufeng Rd., Wufeng, Taichung, 41354, Taiwan