Anita Maya Sutedja, Emiko Yanase, Irmanida Batubara, Dedi Fardiaz, Hanifah Nuryani Lioe
Red kidney beans (Phaseolus vulgaris L.) contain bioactive compounds that are known to exhibit antidiabetic effects via inhibition of α-glucosidase. However, information on the nonpolar components that exhibit antidiabetic activity is limited. Here, we report the isolation and structure determination of components with α-glucosidase inhibitory activity, which were obtained from the hexane extract of red kidney beans. Triacylglycerols (TAGs) were identified as the major components exhibiting inhibitory activity against α-glucosidase. The chemical structure of TAGs was determined by a combination of GC-MS and UPLC-MS/MS. The primary TAGs identified were LnLnLn (trilinolenin) and LnLLn (1,3-dilinolenoyl-2-linoleoyl glycerol). The major fatty acids present in these TAGs were α-linolenic acid (ω-3) and linoleic acid (ω-6). These TAGs were also found to inhibit the α-glucosidase activity in a similar fashion as acarbose. These results suggest that TAGs have potency as antidiabetics and support the potential suitability of red kidney beans for diabetes treatment. © 2019, © 2019 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
Faculty of Applied Biological Sciences, Graduate School of Applied Biological Sciences, Gifu University, Gifu City, Japan; Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, Bogor Agricultural University, Bogor, Indonesia; Department of Food Technology, Faculty of Agricultural Technology, Widya Mandala Catholic University, Surabaya, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Science, Bogor Agricultural University, Bogor, Indonesia; Tropical Biopharmaca Research Center, IPB University, Bogor, Indonesia