His Lin, Ting-Yun Lin, Jer-An Lin, Kuan-Chen Cheng, Shella Permatasari Santoso, Chun-Hsu Chou, Chang-Wei Hsieh
Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiaging-related skincare products. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City, 40227, Taiwan; Graduate Institute of Food Safety, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City, 40227, Taiwan; Institute of Biotechnology, National Taiwan University, Taipei, 10617, Taiwan; Graduate Institute of Food Science Technology, National Taiwan University, Taipei, 10617, Taiwan; Department of Optometry, Asia University, 500, Lioufeng Rd., Wufeng, Taichung City, 41354, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung City, 406040, Taiwan; Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya, 60114, Indonesia; Department of Chemical Engineering, National Taiwan University of Science and Techology, Keelung Rd. 43, Daan Dist, Taipei, 10607, Taiwan; Dr Jou Biotech Co., Ltd., No. 21, Lugong S. 2nd Rd., Lukang Township, Changhua, 505, Taiwan