N. Kusumawati, T.E.W. Widyastuti, I. Kuswardani
This study was carried out to measure the ability of various Na-alginate concentration to protect an encapsulated Lactobacillus acidophilus FNCC 0051 in the synbiotic candidate containing 3% (w/v) of isomalt as prebiotic during storage. The viability of encapsulated Lactobacillus acidophilus FNCC 0051, beads characteristics (diameter and texture of beads), the count of released cells, pH and total acid of milk as beads carrier showed a significant (α = 0.05) difference. Higher concentration of Na-Alginate resulted in higher effectiveness of alginate matrix protection to the encapsulated Lactobacillus acidophilus FNCC 0051 up to 20 days of storage time. Matrix consisting of 2% alginate and 3% isomalt can produce beads with high cell viability in the amount of 9,3 log cfu/g for 20 days at 5°C. This study indicated that alginate combined with isomalt can be an effective matrix protect the bacterial cells during storage.
Agricultural Technology Faculty, Widya Mandala Surabaya Catholic University, Surabaya, East Java, 60265, Indonesia