Vania Bundjaja, Shella Permatasari Santoso, Artik Elisa Angkawijaya, Maria Yuliana, Felycia Edi Soetaredjo, Suryadi Ismadji, Aning Ayucitra, Chintya Gunarto, Yi-Hsu Ju, Ming-Hua Ho
Bacterial contamination on external wounds is known to be a factor that prevents wound healing and triggers tissue damage. Hydrogel-dressings with antibacterial activity is a useful medical device to avoid this contamination, wherein the antibacterial activity can be provided via incorporation of silver nanoparticles (AgNPs). Contrary to the conventional two-step preparation of an AgNPs-loaded hydrogel (AgNPs@hydrogel), this work aims to establish a new and facile synthesis method employing the adsorption principle. Once AgNO3 adsorbed into active sites of the hydrogels, in situ reductions using NaBH4 was employed to produce AgNPs@hydrogel. The effect of surfactant addition on the AgNO3 loading and the antibacterial activity of the resulting hydrogel dressing was investigated. The outcome of this work indicates that the addition of rarasaponin not only can increase the loading of AgNPs on cellulose carbamate hydrogel (CCH) but also significantly enhance the antibacterial activity of the resulted hydrogel-dressing. Superior to the other studied surfactant, the loading capacity (LC) of AgNPs is found to be 10.15, 9.94, and 7.53 mg/g for CCH modified with rarasaponin, CTAB, and Tween80, respectively. These findings conclude that the addition of surfactant, especially rarasaponin, can effectively improve the loading of AgNPs onto hydrogel-dressing via adsorption and promote the antibacterial activity. Furthermore, the cytotoxic test shows that the hydrogel-dressings have good biocompatibility toward skin fibroblast cells. © 2020 Elsevier B.V.
Chemical Engineering Department, National Taiwan University of Science and Technology, #43, Sec. 4, Keelung Rd., Taipei, 10607, Taiwan; Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, #43, Sec. 4, Keelung Rd., Taipei, 10607, Taiwan; Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, #37 Kalijudan Rd., Surabaya, 60114, East Java, Indonesia; Taiwan Building Technology Center, National Taiwan University of Science and Technology, #43, Sec. 4, Keelung Rd, Taipei, 10607, Taiwan